Journal of the Korean Home Economics Association 2001;39(4):49-59.
홍화색소의 일반추출과 셀룰라아제추출의 비교연구
신인수1, 홍경옥1, 오태광2
원광대학교 생활과학대학 의상학과1, 생명공학연구원 환경생물소재연구실2
Comparison Studies between Conventional Hot Water and Cellulase Extraction for Safflower Dyestuff
신인수1, 홍경옥1, 오태광2
ABSTRACT
Natural red and yellow dyestuff was extracted from safflower (Carthamus tinctorius Linnaeus) by a new process of cellulase extraction compared with the conventional hot water extraction. Dyestuffs were extracted from safflower easily and repeatedly by means of cellulose as safflower cell wall destroyer. It means that new dyestuff extraction by cellulase improves not only yields of dyestuff from safflower successfully but also the rate of repetition of extraction. From the above experiments, the conclusions of this study were summarized as follows. 1. The optimum conditions of dyestuff extraction from safflower by general extraction method were that the solvent was the water of pH 6.0 on yellow dyestuff and 3% K2CO3 solution on red dyestuff, extraction temperature was 55℃, and extraction time was 30 min. 2. Among various cellulase, the NOVO cellulase was the best cell wall destroyer of safflower and finally produced the largest amount of dyestuff from safflower by cellulase extraction method. 3. The optimum conditions of dyestuff extraction by cellulase extraction method were conducted on 10 unit of cellulase per gram of safflower at 100㎖ water of pH 5.0 at 50℃ for 30 min.
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